Izac, J.
, Kwee, E.
, Gaigalas, A.
and Wang, L.
(2024),
Quantitative and Standardized Pseudovirus Neutralization Assay for COVID-19, Humana Press/Springer, Totowa, NJ, [online], https://doi.org/10.1007/978-1-0716-3738-8_11, https://tsapps.nist.gov/publication/get_pdf.cfm?pub_id=956266
(Accessed May 15, 2025)
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Quantitative and Standardized Pseudovirus Neutralization Assay for COVID-19
Published
Author(s)
, , Adolfas Gaigalas,
Abstract
COVID-19 is a global pandemic caused by the highly infectious SARS-CoV-2 virus. Efforts to combat SARS-CoV-2 infection include mass vaccination and development of monoclonal and convalescent plasma therapeutics that require precise measurements of correlative, functional neutralizing antibodies that prevent virus infection. Developing rapid, safe, easy-to-use, and high-quality neutralization assays are essential for the success of the massive effort. Here, we developed a vesicular stomatitis virus-based neutralization assay that was capable of quantifying varying degrees of neutralization in patient serum samples. This assay has two detection readouts, flow cytometry and live cell imaging. The two readout methods produced consistent values of all 50% neutralization titers, further enhancing measurement confidence on the assay. Moreover, the use of available reference standards such as WHO IS enables quantification and standardization of the pseudovirus neutralization assay with neutralizing antibody titers measured in the unit of IU/mL. Quantitative and standardized neutralization assays are critical for reliable efficacy evaluation and comparison of numerous vaccines and therapeutics.Citation
Flow Cytometry Protocols, 5th Edition
Publisher Info
Humana Press/Springer, Totowa, NJ
Pub Type
Books
Citation
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Created April 1, 2024, Updated December 3, 2024